Instead, Singh would be staying at the Willard Intercontinental Hotel, located three blocks away from the White House.
The decision has been taken at the request of India, those familiar with the move said.
Such a decision was primarily taken keeping in view the inconvenience officials and other dignitaries calling on the Prime Minister might face, due to the strict security regulations at the Blair House.
Informed sources said nothing should be read out into this decision.
The Prime Minister, who is set to meet President Barack Obama on November 24, had stayed at the Blair House in 2005 when he was the State Guest of the then US President, George W Bush.
Singh's US trip is the first state visit of the Obama Administration.
The Guest Book of the Blair House has the signature of the first Prime Minister, Pandit Jawaharlal Nehru, and his daughter Indira Gandhi. The signature is dated December 19, 1956.