The Australian Open was hit by two more withdrawals on Saturday with 11th seed Paula Badosa and home hope Ajla Tomljanovic both injured.
The first Grand Slam of the year has seen several players pull out ahead of the opening day on Monday, including the injured men's number one Carlos Alcaraz and Naomi Osaka, who is pregnant.
Spain's former world number two Badosa became the latest high-profile casualty because of a thigh injury picked up this week in a warm-up tournament.
"Bad news... during the quarter-final match in Adelaide I hurt myself and after receiving the results I will have to be out for a few weeks," the 25-year-old tweeted.
"Sad to miss Australian Open this year, really going to miss it.
"I will do everything I can to return as soon as possible."
Also ruled out on Saturday was Australia's 35th-ranked Tomljanovic because of a knee injury.
They will be replaced by Brazil's Laura Pigossi and Leolia Jeanjean of France.